Urinalysis

Mid-stream urine (MSU)

• Not first or last part of urine that comes out, reduces contamination with bacteria
• Obtain sample from middle of bladder
• Urine does not normally have any bacteria
• Confirm diagnosis of a urine infection
• Symptoms of urine infection are pain when passing urine and doing it frequently
• Decide the best antibiotic to use

Collection

• Wash hands and genitals
• Women - hold open the entrance to vagina
• Men - pull back foreskin
• Pass some urine into toilet, then catch urine in clean bottle
• One enough urine is collected pass the rest of the urine into toilet
• Avoid touching genitals on bottle

MSU pots

• Yellow top pot without preservative - 2 hours before bacteria overgrows and unsuitable for testing
• Collect within 2 hours is best and get to GP practice before 11AM

Catheter specimen urine (CSU)

• Bacteriological examination if urinary tract infection is suggested by symptoms
• Non-touch, aseptic technique
• Taken from sampling port on drainage bag

Equipment

• Plastic apron
• Non-sterile gloves
• Sterile 10ml syringe
• 2x 70% alcohol swaps
• Disposable clamp
• Specimen containers
• Laboratory request form
• Detergent wipe

Collection

1. Put on apron
2. Decontaminate hands
3. If no urine in catheter bag tubing, clamp tubing 2-3 inches below the sampling port using a scissor style clamp
4. Decontaminate hands
5. Apply gloves
6. Clean sampling port
7. Attach syringe to sampling port to aspirate urine
8. Clean sampling port after removing syringe
9. Place urine in yellow top specimen container in view of using the green topped vacutainer before sending to laboratory
10. Remove clamp from catheter bag tubing and decontaminate
11. Remove gloves and apron
12. Wash hands
13. Label specimen
14. Complete laboratory request stating clinical symptoms